Paul A. Luciw, PhD
Viral Pathogenesis and Therapy (HIV and AIDS)
Dr. Paul Luciw is a virologist with extensive experience in many aspects of virology, cell biology, and molecular biology. The main emphasis of his research is on viruses that establish persistent infection; these include retroviruses that cause immunodeficiency and herpesviruses associated with cancer. His recent translational research has focused on the development of novel technologies for studies of infectious diseases and cancer.
For studies on simian immunodeficiency virus (SIV), a major aim of Dr. Luciw's research is to identify viral determinants of pathogenesis in the non-human primate model (i.e., rhesus monkeys) for AIDS. This research aims to define functional domains on viral proteins and to analyze molecular mechanisms regulating viral transcription in latency and reactivation. He is testing novel pharmacologic approaches targeted at inducing virus (SIV or HIV) from latent cell reservoirs. Coupled with highly active antiretroviral therapy, induction of latent virus by compounds that activate specific transcription factors and remodel chromatin, could lead to elimination of virus from an infected individual. Dr. Luciw's collaborative studies on the oncogenic herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV), are focused on viral genes regulating signal transduction and transcription and on post-translational modifications of chromatin proteins during viral infection. The goal is to understand the molecular mechanisms by which this oncogenic herpesvirus alters cell functions to favor viral replication and mediate tumorigenesis.
For translational research of infectious diseases, Dr. Luciw has developed multiplex immunoassay technology for detection of antibodies to multiple infectious agents and analysis of host response immunomodulatory proteins to infection. To study host responses to Mycobacterium tuberculosis, his laboratory has used multiplex immunoassays for studies of cytokines, chemokines, and inflammatory mediators as well as antibodies to multiple antigens of this pathogen in nonhuman primates and humans. Importantly, these robust multiplex assays have strong potential for significantly improving diagnosis and prognosis of tuberculosis. He has also applied this novel immunoassay for detection of antibodies to multiple infectious agents of mouse (UC Davis Mouse Biology Program) and rhesus monkey (California Primate Center). For application to studies on mechanisms of tumorigenesis, Dr. Luciw has been developing multiplex immunoassay systems that simultaneously detect multiple cell signaling molecules (phosphotyrosine kinsease and substrates, phosphoserine/threonine kinsese and substrates, transcription factors). This system enables simultaneous analysis of multiple signal transduction pathways and multiple components of these pathways in cancer cell line models and tumors. His laboratory also performs multiplex analysis of protein targets in plasma of cancer patients, with the goal of defining rapid and effective methods for diagnosis and prognosis. These plasma targets include include biomarkers associated with various stages of tumorigenesis (growth factors, proteinases, oncogenes, angiogenesis factors, etc.).
Gardner, M.B. and P.A. Luciw (2008) Macaque Models for Human Infectious Diseases. Institute for Laboratory Animal Research. 49:220-255.
Krishnan, V.V., I.H. Khan, and P.A. Luciw (2009) Multiplexed microbead immunoassays by flow cytometry for molecular profiling: Basic concepts and proteomics applications. Current Reviews in Biotechnology. 29:29-43.
Izumiya Y., C. Izumiya, D. Hsia, T.J. Ellison, P.A. Luciw, and H-J. Kung. (2009) NF-kappaB serves as a cellular sensor of KSHV latency and negatively regulates the viral transactivator K-Rta. J. Virol. 83:4435-46.
Khan I., J. Zhao, P. Ghosh. M. Ziman, C. Sweeney, H-J. Kung, and P. Luciw (2009) Multiplex microbead auspension arrays for simultaneous analysis of ErbB family of receptor tyrosine kinases: activation and dimerization studies in breast cancer cells. ASSAY and Drug Devel. Technologies 8: 27-36.
North, T.W., J. Higgens, J.D. Deere, T.L. Hayes, A. Villalabos, L. Adamson, B.L. Shacklett, R.F. Schinazi, and P.A. Luciw. (2010) Viral sanctuaries during highly active antiretroviral therapy in a nonhuman primate model for AIDS. J. Virol. 84: 2913-2922.
Chang P-C.,Y. Izumiya,C-Y. Wu, L.D. Fitzgerald, M. Campbell, T.J. Ellison, K.S. Lam, P.A. Luciw, and H-J. Kung. (2010) Kaposi’s sarcoma associated herpesvirus (KSHV) encodes a SUMO E3 ligase which is SIM-dependent and SUMO2/3-specific. J. Biol. Chem. 285: 5266-5273.
Deere, J.D., J. Higgins, E. Cannavo, A. Villalobos, L. Adamson, R.F. Schinazi, P.A. Luciw, and T.W. North. (2010) Low-level viremia persists despite suppressive highly active antiretroviral therapy in rhesus macaques infected with RT-SHIV. PLoS One. 5(7):e11640.
Campbell, M., W-R. Lie, J. Zhao, D. Hayes, J. Mistry, H-J. Kung, P.A. Luciw, and I.H. Khan. (2010) Multiplex analysis of Src family kinase signaling by microbead suspension arrays. ASSAY and Drug Devel. Technologies. 8:488-96.
Ravindran, R., I.H. Khan, V.V. Krishnan, M. Ziman, J.K. Frasier, R. Bates, S.M. Griffey, J.R. Fahey, L.V. Kendall, P.A. Luciw. (2010) Validation of multiplex microbead immunoassay for simultaneous serodiagnosis of multiple infectious agents in laboratory mice. J. Immunol. Methods. 363:51-59.
Chang P.C., L.D. Fitzgerald, D.A. Hsia, Y. Izumiya, C.Y. Wu, W.P. Hsieh, S.F. Lin, M. Campbell, K.S. Lam, P.A. Luciw, C.G. Tepper, H.J. Kung. (2011) Histone demethylase JMJD2A regulates KSHV replication and is targeted by a viral transcriptional factor. J. Virol. 85:3283-93.
Luciw, P.A., K.L. Oslund, X-W. Yang, L. Adamson, R. Ravindran, D. Canfield, R. Tarara, L. Hirst, M. Christensen, N. W. Lerche, H. Offenstein, D. Lewinsohn, F. Ventimiglia, L. Brignolo, E.R. Wisner, and D.M. Hyde (2011) Stereologic Analysis of Bacterial Load and Lung Lesions in Nonhuman Primates (Rhesus Macaques) Experimentally Infected with Mycobacterium tuberculosis. Am J Physiol - Lung Cell Mol Physiol. 301:L731-8.
Khan I.H., R. Ravindran, V.V. Krishnan, I. Nawaz Awan, S.K.A. Rizvi, M.A.N. Saqib, M.I. Shahzad, S. Tahseen, G. Ireton, C. Goulding, P. Felgner, K. DeRiemer, A. Khanum, and P.A. Luciw. (2011) Plasma antibody profiles as diagnostic biomarkers for tuberculosis. Clin Vaccine Immunol. 18:2148-53.
Murphy B., N. Vapniarsky., C. Hillman, D. Castillo, S. McDonnel, Moore P., P.A. Luciw and E.E. Sparger. (2012) FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes in vivo during the asymptomatic phase of infection. (Accepted by Retrovirology)
Staff Scientist, California National Primate Research Center
Director of the Clinical Proteomics Core, UC Davis
Executive Committee, UC Davis Optical Biology Facility
UC Davis Biotechnology Advisory Board
UC Davis Microbiology Graduate Group - Executive Committee
UC Davis Biochemistry and Molecular Biology Graduate Group
UC Davis Genetics Graduate Group
UC Davis Comparative Pathology Graduate Group
UC Davis Cancer Center, Member
Targeted Action Group for Vaccines, UC San Francisco
Editorial Board, Journal of Virology
Editorial Board, AIDS Research and Human Retroviruses